Fifteen years of APC/cyclosome: a short and impressive biography.
نویسندگان
چکیده
The APC/C (anaphase-promoting complex/cyclosome) discovered exactly 15 years ago by Avram Heshko and Marc Kirschner is by far the most complex ubiquitin ligase discovered so far. The APC/C is composed of roughly a dozen subunits and measures a massive 1.5 MDa. This huge complex, as well as its multiple modes of regulation, boasts impressive evolutionary conservation. One of its most puzzling features is its split personality: regulation of mitotic exit events on the one hand, and its ongoing activity during G(1)-phase, G(0)-phase and in terminally differentiated cells. The present short review is intended to provide a basic description of our current understanding of the APC/C, focusing on recent findings concerning its role in G(1)-phase and in differentiated cells.
منابع مشابه
Editorial Report of fifteen years contributions to radiological sciences: Future directions and prospects
At the close of the 20th century, fundamental discoveries changed broadly the worlds of physics, biology and medicine. The rapid advancements achieved during recent years, mainly due to revolutionary methodological improvements, have led to an unparalleled explosion of information; often appear to overshadow the earlier works. However as more basic discoveries are made these separate scientific...
متن کاملFizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.
The Xenopus homologue of Drosophila Fizzy and budding yeast CDC20 has been characterized. The encoded protein (X-FZY) is a component of a high molecular weight complex distinct from the APC/cyclosome. Antibodies directed against FZY were produced and shown to prevent calmodulin-dependent protein kinase II (CaMKII) from inducing the metaphase to anaphase transition of spindles assembled in vitro...
متن کاملThe anaphase-promoting complex/cyclosome: APC/C.
The major ubiquitin ligase required for mitosis is the anaphase-promoting complex/cyclosome (APC/C). This unusually complex E3 ubiquitin ligase targets cell-cycle-related proteins such as cyclins and securin for degradation by the proteasome in mitosis and meiosis. The APC/C is regulated by phosphorylation, as well as by various activators and inhibitors that alter its substrate specificity at ...
متن کاملVisualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C)
The anaphase promoting complex or cyclosome (APC/C) is a large multi-subunit E3 ubiquitin ligase that orchestrates cell cycle progression by mediating the degradation of important cell cycle regulators. During the two decades since its discovery, much has been learnt concerning its role in recognizing and ubiquitinating specific proteins in a cell-cycle-dependent manner, the mechanisms governin...
متن کاملMitosis: Short-Circuiting Spindle Checkpoint Signaling
The spindle checkpoint forms an intricate signaling circuit to sense unattached kinetochores, to inhibit the anaphase-promoting complex/cyclosome (APC/C), and to delay anaphase onset. Using clever genetic experiments in the budding yeast, Lau and Murray define the endpoint of checkpoint signaling and provide key mechanistic insights into checkpoint inhibition of APC/C.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Biochemical Society transactions
دوره 38 Pt 1 شماره
صفحات -
تاریخ انتشار 2010